Cell Therapy Media and Accessory

Irvine Scientific® offers the PRIME-XV® Media Portfolio comprising xeno- and serum-free media for utilization in cell therapy and regenerative medicine.

Validated culture optimized expansion media for T cells, MSCs, Neutral Cells, Amniotic Fluid Stem Cells (AFSCs), Cancer Stem/Initiating Cells or human/murine Hematopoietic Cells (HPCs) and differentiation media for osteogenesis, adipogenesis and chondrogenesis are manufactured under full cGMP conditions and fulfill CE regulations. Cell cryopreservation solutions, various substrates and animal-component-free recombinant growth factors complete the portfolio.

PRIME-XV® Media and Accessory for T Cells

CATALOG NO. DESCRIPTION STORAGE SIZE
91146 PRIME-XV® Dendritic Cell Maturation CDM 4°C 500 ml
91154 PRIME-XV® T Cell CDM 4°C 1 l
91141 PRIME-XV® T Cell Expansion XSFM 4°C 1 l
95112 PRIME-XV® Recombinant Human GM-CSF ACF -20°C 20 μg
95117 PRIME-XV® Recombinant Human TNF-alpha ACF -20°C 10 μg
95118 PRIME-XV® Recombinant Human IL-2 ACF -20°C 10 μg
91113 PRIME-XV® Recombinant Human IL-3 ACF -20°C 10 μg
91114 PRIME-XV® Recombinant Human IL-4 ACF -20°C 20 μg
95121 PRIME-XV® Recombinant Human IL-6 ACF -20°C 20 μg

NEW: PRIME-XV® Dendritic Cell Maturation CDM

Chemically-defined, animal component-free medium for cultivation, maturation and maintenance of monocyte-derived dendritic cells for immunogenic applications

  • Optimized for differentiation of monocytes into immature dendritic cells (iDCs) and subsequent maturation of mDCs
  • Achieve high yields of DCs with the desired characteristics and morphology
  • Maintains potency of dendritic cells for T cell stimulation
  • Ready-to-use, just supplement with desired cytokine cocktails
  • cGMP and chemically-defined - facilitate transfer from research to clinic

First data with this new chemically-defined medium (CDM) are published in a POSTER available in the download area below.

NEW: PRIME-XV® T Cell CDM

Chemically-defined, animal component-free medium for T cell culture optimized to deliver consistent growth while maintaining T cell functionality and potency

  • Supports vigorous T cell growth in plates, cell culture bags, and in the G-Rex® while maintaining functionality
  • Provides lot-to-lot consistency
  • Removes the effects of undefined components on T cell phenotypes
  • Supports polarization to targeted T cell types such as Th1 and T regulatory cells
  • Traceability documentation provided including Certificates of Analysis, Certificates of Origin, and a Drug Master File (DMF) filed with the US FDA
  • Extensive QA testing including functionality, sterility, and endotoxin

PRIME-XV® T Cell Expansion XSFM

T cell expansion xeno-free, serum-free medium

  • Optimized for the activation and expansion of human T lymphocytes
  • Maintains T cell potency
  • Supports expansion of memory T cells
  • Serum-free and xeno-free formulation reduces risk of contamination and provides better lot-to-lot consistency
  • Drug Master File (DMF) registration
  • Contains gentamicin

Recombinant Groth Factors / Cytokines

Produced entirely animal-component-free (ACF) in an E.coli expression system. Please inquire for the entire range!

PRIME-XV Dendritic Cell Maturation CDM- Poster (2017) (PDF, 665 KB)
PRIME-XV Dendritic Cell Maturation CDM- Flyer (2017) (PDF, 480 KB)
PRIME-XV T Cell CDM- Poster (2017) (PDF, 1,8 MB)
PRIME-XV T Cell CDM- Flyer (2017) (PDF, 481 KB)

Find more detailed information on the Irvine Scientific homepage: Dendritic Cells or T Cells

PRIME-XV® Media and Accessory for Mesenchymal Stem/Stroma Cells (MSCs)

CATALOG NO. DESCRIPTION STORAGE SIZE
91135 PRIME-XV® MSC Expansion SFM -20°C 250 ml
91149 PRIME-XV® MSC Expansion XSFM -20°C 250 ml/ 1 l
91132 PRIME-XV® Osteogenic Differentiation SFM -20°C 100 ml
91137 PRIME-XV® Adipogenic Differentiation SFM -20°C 100 ml
91138 PRIME-XV® Chondrogenic Differentiation XSFM -20°C 100 ml
31001 PRIME-XV® MatrIS F -20°C 200 μg
31002 PRIME-XV® Human Fibronectin -20°C 1 mg
31139 PRIME-XV® FreezIS -20°C 10 ml/ 100 ml
91140 PRIME-XV® FreezIS DMSO-free -20°C 10 ml/ 100 ml

PRIME-XV® MSC Expansion SFM

Human mesenchymal stromal/stem cell (MSC) expansion serum-free medium

  • Outperforms leading competitors and serum-containing media in expansion while maintaining MSC characteristics and multipotency
  • Supports MSCs derived from bone marrow, adipose tissue and umbilical cord
  • Available as a complete, ready-to-use medium
  • Requires little to no adaptation from serum-containing medium

PRIME-XV® MSC Expansion XSFM

Human mesenchymal stromal/stem Cell (MSC) expansion xeno-free / serum-free medium

  • Maintains MSC quality after extended culture
  • Supports MSCs derived from human adipose, human bone marrow, and human umbilical cord
  • Xeno-free and serum-free formulation for lot-to-lot consistency
  • Drug Master File (DMF) registration

PRIME-XV® Osteogenic Differentiation SFM

Osteogenic differentiation serum-free medium

  • Supports robust induction of osteogenesis from human adipose-derived MSCs and human amniotic fluid stem cells (AFSCs)
  • Classical staining method and immunocytochemistry demonstrate the presence of osteoblasts and osteocytes
  • Available as a complete, ready-to-use medium

PRIME-XV® Adipogenic Differentiation SFM

Adipogenic differentiation serum-free medium

  • Supports robust induction of osteogenesis from human adipose-derived MSCs and human amniotic fluid stem cells (AFSCs)
  • Classical staining method and immunocytochemistry demonstrate the presence of osteoblasts and osteocytes
  • Available as a complete, ready-to-use medium

PRIME-XV® Chondrogenic Differentiation XSFM

Chondrogenic differentiation xeno-free serum-free medium

  • Supports robust induction of chondrogenesis from human mesenchymal stem/ stromal cells (MSCs)
  • Classical staining method and immunocytochemistry demonstrate the presence of chondrocytes
  • Available as a complete, ready-to-use medium
PRIME-XV MSC Expansion XSFM- Flyer (2017) (PDF, 430 KB)

PRIME-XV® Media and Accessory for Neural Cells

CATALOG NO. DESCRIPTION STORAGE SIZE
91201 PRIME-XV® Neural Basal Medium 4°C 500 ml
91131 PRIME-XV® NPC Expansion XSFM -20°C 250 ml
91142 PRIME-XV® IS21 Supplement (50X) -20°C 10 ml

PRIME-XV® Media for hHPCs, mHPCs, AFSCs and Tumorspheres

CATALOG NO. DESCRIPTION STORAGE SIZE
91211 PRIME-XV® Hematopoietic Cell Basal XSFM 4°C 500 ml
91206 PRIME-XV® Mouse Hematopoietic Cell Basal Medium 4°C 500 ml
91133 PRIME-XV® AFSC Expansion Medium -20°C 250 ml
91130 PRIME-XV® Tumorsphere SFM -20°C 100 ml

NEW: PRIME-XV® Hematopoietic Cell Basal XSFM

Xeno-free, serum-free basal medium for human hematopoietic progenitor cell culture

  • Optimized to support vigorous expansion of hematopoietic progenitor cells while maintaining high Total Nucleated Cell (TNC) count and percentages of CD34+, when supplemented with a cocktail of cytokines
  • Provides lot-to-lot consistency
  • Manufactured to be scalable and to facilitate transfer from research to clinic
  • Drug Master File (DMF) registration
PRIME-XV Hematopoietic Cell Basal XSFM- Flyer (2017) (PDF, 651 KB)

PRIME-XV® Attachement Substrates

CATALOG NO. DESCRIPTION STORAGE SIZE
31001 PRIME-XV® MatrIS F (rHuman Matrix Protein) -20°C 200 μg
31002 PRIME-XV® Human Fibronectin -20°C 1 mg

PRIME-XV® Cell Cryopreservation Solutions

The ability to preserve and thaw primary and stem cells has tremendous potential in allogenic and autologous transplants for clinical therapeutic applications. Irvine Scientific's cryopreservation products are formulated as chemically-defined, protein-free solutions that are manufactured under GMP.

CATALOG NO. DESCRIPTION STORAGE SIZE
91139 PRIME-XV® FreezIS 4°C 10 ml/ 100 ml
91140 PRIME-XV® MSC FreezIS DMSO-free 4°C 10 ml/ 100 ml

PRIME-XV® FreezIS

Protein-free, chemically-defined cryogenic preservation solution for cells and tissues

  • Enables preservation of a variety of cell types at -80°C to -196°C environments
  • Maintains cell surface marker expression of cells post-thaw
  • No additional components are needed
  • Drug Master File (DMF) registration

NEW: PRIME-XV® MSC FreezIS DMSO-Free

DMSO-free, chemically-defined cryogenic preservation solution for human mesenchymal stem/stromal cells

  • Comparable post-thaw cell viability as solutions containing dimethyl sulfoxide (DMSO)
  • Maintain potency of mesenchymal stem/stromal cells (MSCs) throughout cryopreservation
  • Eliminate risk of DMSO in MSC applications
  • Animal component-free, chemically-defined solution
  • Enables cell preservation at -80°C to -196°C environments
  • Drug Master File (DMF) registration

Downloads

PRIME-XV® Portfolio - Product List (PDF, 668 KB)

Find more detailed information on the Irvine Scientific homepage: Cell Therapy Solutions

PRIME-XV® products are usually processed under cGMP conditions and for research or further manufacturing use only.

Abbreviations:
ACF ≡ Animal Component-Free
AFSC ≡ Amniotic Fluid Stem Cell
CDM ≡ Chemically Defined Medium
HPC ≡ Hematopoietic Progenitor Cell
MSC ≡ Mesenchymal Stem/Stroma Cell
NPC ≡ Neural Progenitor Cell
SFM ≡ Serum-Free Medium
XSFM ≡ Xeno-Free / Serum-Free Medium