CellExpand

Suspension Expansion Culture (SEC) Reagents

For expansion of lympho-hematopoietic cells in suspension

  • For 96-well, 24-well, 12-well or 6-well plate suspension cultures or large culture flask volumes
  • Easy and rapid high-content screening for proliferation and differentiation processes
  • Evaluate the effects of drugs, xenobiotics or other agents on the differentiation/maturation process

CellExpand is available as master mix for various lympho-hematopoietic cell populations [growth factors contained therein]:

Stem cells

  • SC-HPP 1 [IL-3, IL-6, SCF, TPO, Flt3-L]
  • SC-HPP 2 [EPO, GM-CSF, IL-2, IL-3, IL-6, IL-7, SCF, TPO, Flt3-L]
  • SC-GEMM 1 [EPO, GM-CSF, IL-3, IL-6, SCF, TPO, Flt3-L]
  • SC-GEMM 2 [EPO, GM-CSF, IL-3, IL-6, SCF, TPO]
  • SC-GEMM 3 [EPO, GM-CSF, G-CSF, IL-3, IL-6, SCF, TPO]
  • SC-GEM 1 [EPO, GM-CSF, IL-3, IL-6, SCF]
  • SC-GEM 2 [EPO, GM-CSF, IL-3, SCF]

Progenitor cells

  • P-BFU 1 [EPO, IL-3, SCF]
  • P-BFU 2 [EPO (high dose)]
  • P-GM 1 [GM-CSF, IL-3, SCF]
  • P-GM 3 [GM-CSF]
  • P-Mk 1 [TPO, IL-3, SCF]
  • P-Tcell [IL-2 (also requires addition of co-stimulator)]
  • P-Bcell [IL-7]

Use of CellExpand

  • High throughput flow cytometry
  • Cell signaling studies
  • DNA, RNA analysis and other molecular biology methods
  • Mechanism of Action (MOA) studies
  • Evaluate the effects of drugs, xenobiotics or other agents on the differentiation/maturation process
  • Gene transfection and targeting studies, e.g. CRISPR-Cas9
  • CellExpand Master Mixes are liquid and thereby allow cell interactions providing:
    • Shorter lag time to initiate proliferation
    • More rapid culture completion
    • Greater sensitivity

Specifications

Human (Cat.-No. below), Non-Human Primate (Macaca fascicularis (Cynomologus) and Macaca mulatta (Rhesus)), Canine, Rat or Mouse

Low serum formulations (below) are available for the above listed species in 40 ml or bulk quantities; Serum-free formulations are available for Human, Non-Human Primate and Mouse cells only.

Use HemoGro-LS or HemoGro-HPSF for controls (no growth factors).

For research use only. Not for clinical diagnostic use.

CellExpand Master Mix / SEC

Catalog no. Description Storage Size
       
SEC-0-40H CellExpand without growth factors -20°C 40 ml
SEC-HPP1-40H CellExpand for SC-HPP1 / HPP-SP1 -20°C 40 ml
SEC-HPP2-40H CellExpand for SC- HPP2 / HPP-SP2 -20°C 40 ml
SEC-GEMM1-40H CellExpand for SC-GEMM1 / CFC-GEMM1 -20°C 40 ml
SEC-GEMM2-40H CellExpand for SC-GEMM2 / CFC-GEMM2 -20°C 40 ml
SEC-GEMM3-40H CellExpand for SC- GEMM3 / CFC-GEMM3 -20°C 40 ml
SEC-GEM1-40H CellExpand for SC- GEM1 / CFC-GEM1 -20°C 40 ml
SEC-GEM2-40H CellExpand for SC-GEM2 / CFC-GEM2 -20°C 40 ml
SEC-BFU1-40H CellExpand for P-BFU1 / BFU-E1 -20°C 40 ml
SEC-BFU2-40H CellExpand for P-BFU2 / BFU-E2 -20°C 40 ml
SEC-GM1-40H CellExpand for P-GM1 / GM-CFC1 -20°C 40 ml
SEC-GM3-40H CellExpand for P-GM3 / GM-CFC3 -20°C 40 ml
SEC-Mk1-40H CellExpand for P-Mk1 / MK-CFC1 -20°C 40 ml
SEC-T-40H CellExpand for P-Tcell / T-CFC -20°C 40 ml
SEC-B-40H CellExpand for P-Bcell / B-CFC -20°C 40 ml

This catalog numbers are for Human cells / Low serum formulation; available as well for Primate, Canine, Rat or Mouse.
Serum-free formulations are available for Human, Primate and Mouse cells.

Please find Terminology / Synonyms of hematopoietic stem, progenitor and precursor cells here