Methylcellulose CFU-Assays

Classical assays for hematopoietic cell processing

These reagents and assays allow the clonal detection in methylcellulose. Designed for hematopoietic stem- and progenitor cells they provide an indication for erythroid, neutrophil and platelet production.

  • ColonyGro: Methylcellulose CFU Master Mixes for 44 x 1ml cultures performed in duplicate
  • CAMEO-4: Miniaturized CFU complete assay kits, including culture plates for 50 x 0.1ml cultures performed in quadruplicate

Advantages of Preferred Cell Systems Methylcellulose CFU-Assays

  • Easy handling: due to the optimized blend of the master mix it can simply be applied with a positive displacement pipette
  • Fast results in 10-12 days: media formulation allows increased growth of colonies
  • Economical use of cells: the miniaturized CFU-assay kit CAMEO-4 requires only 1/10 the amount of target cells compared to traditional assays

ColonyGro

Methylcellulose colony-forming assay reagents

Catalog no. Description Storage Size
CG-0-100H ColonyGro without growth factors -20°C 100 ml
CG-HPP1-100H ColonyGro for SC-HPP1 / HPP-SP1 -20°C 100 ml
CG-HPP2-100H ColonyGro for SC-HPP2 / HPP-SP2 -20°C 100 ml
CG-GEMM1-100H ColonyGro for SC-GEMM1 / CFC-GEMM1 -20°C 100 ml
CG-GEMM2-100H ColonyGro for SC-GEMM2 / CFC-GEMM2 -20°C 100 ml
CG-GEMM3-100H ColonyGro for SC-GEMM3 / CFC-GEMM3 -20°C 100 ml
CG-GEM1-100H ColonyGro for SC-GEM1 / CFC-GEM1 -20°C 100 ml
CG-GEM2-100H ColonyGro for SC-GEM2 / CFC-GEM2 -20°C 100 ml
CG-GEM3-100H ColonyGro for SC-GEM3 / CFC-GEM3 -20°C 100 ml
CG-BFU1-100H ColonyGro for P-BFU1 / BFU-E1 -20°C 100 ml
CG-BFU2-100H ColonyGro for P-BFU2 / BFU-E2 -20°C 100 ml
CG-E-100H ColonyGro for CFU-E -20°C 100 ml
CG-GM1-100H ColonyGro for P-GM1 / GM-CFC1 -20°C 100 ml
CG-GM2-100H ColonyGro for P-GM2 / GM-CFC2 -20°C 100 ml
CG-GM3-100H ColonyGro for P-GM3 / GM-CFC3 -20°C 100 ml
CG-G-100H ColonyGro for G- CFC -20°C 100 ml
CG-M-100H ColonyGro for M-CFC -20°C 100 ml
CG-Mk1-100H ColonyGro for P-Mk1 / Mk-CFC1 -20°C 100 ml
CG-Mk2-100H ColonyGro for P-Mk2 / Mk-CFC2 -20°C 100 ml
CG-T-100H ColonyGro for P-Tcell / T-CFC -20°C 100 ml
CG-B-100H ColonyGro for P-Bcell / B-CFC -20°C 100 ml

CAMEO-4

Miniaturized methylcellulose colony-forming assay kits

CATALOG NO. DESCRIPTION STORAGE SIZE
KC-0-50H CAMEO-4 without growth factors -20°C 38 ml + 50 plates
KC-HPP1-50H CAMEO-4 for SC-HPP1 / HPP-SP1 -20°C 38 ml + 50 plates
KC-HPP2-50H CAMEO-4 for SC-HPP2 / HPP-SP2 -20°C 38 ml + 50 plates
KC-GEMM1-50H CAMEO-4 for SC-GEMM1 / CFC-GEMM1 -20°C 38 ml + 50 plates
KC-GEMM2-50H CAMEO-4 for SC-GEMM2 / CFC-GEMM2 -20°C 38 ml + 50 plates
KC-GEMM3-50H CAMEO-4 for SC-GEMM3 / CFC-GEMM3 -20°C 38 ml + 50 plates
KC-GEM1-50H CAMEO-4 for SC-GEM1 / CFC-GEM1 -20°C 38 ml + 50 plates
KC-GEM2-50H CAMEO-4 for SC-GEM2 / CFC-GEM2 -20°C 38 ml + 50 plates
KC-GEM3-50H CAMEO-4 for SC-GEM3 / CFC-GEM3 -20°C 38 ml + 50 plates
KC-BFU1-50H CAMEO-4 for P-BFU1 / BFU-E1 -20°C 38 ml + 50 plates
KC-BFU2-50H CAMEO-4 for P-BFU2 / BFU-E2 -20°C 38 ml + 50 plates
KC-E-50H CAMEO-4 for CFU-E -20°C 38 ml + 50 plates
KC-GM1-50H CAMEO-4 for P-GM1 / GM-CFC1 -20°C 38 ml + 50 plates
KC-GM2-50H CAMEO-4 for P-GM2 / GM-CFC2 -20°C 38 ml + 50 plates
KC-GM3-50H CAMEO-4 for P-GM3 / GM-CFC3 -20°C 38 ml + 50 plates
KC-G-50H CAMEO-4 for G-CFC -20°C 38 ml + 50 plates
KC-M-50H CAMEO-4 for M-CFC -20°C 38 ml + 50 plates
KC-Mk1-50H CAMEO-4 for P-Mk1 / Mk-CFC1 -20°C 38 ml + 50 plates
KC-Mk2-50H CAMEO-4 for P-Mk2 / Mk-CFC2 -20°C 38 ml + 50 plates
KC-T-50H CAMEO-4 for P-Tcell / T-CFC -20°C 38 ml + 50 plates
KC-B-50H CAMEO-4 for P-Bcell / B-CFC -20°C 38 ml + 50 plates

CAMEO-4 - Flyer (PDF, 691 KB)

Although methylcellulose CFU-assays are classical assays for this application, please have a look at our special recommendations for time to engraftment after transplantation assays.

Please find Terminology / Synonyms of hematopoietic stem, progenitor and precursor cells here