S9 Liver Extract and Metabolic Activation

Bottles of S9 - Aroclor and PB/BNF induced

S. typhimurium and E. coli, used in Ames test, are prokaryotes and therefore do not exactly reflect the conditions in humans. In mammalian organisms chemical molecules are often metabolized in the liver. This can lead to the generation of mutagenic metabolites.

S9 is a liver extract (e.g. rat, hamster or human) that contains active liver enzymes (P450 activity) simulating the hepatic metabolism in in vitro assays.

The P450 activity in S9 can be increased through induction with pure chemicals like Aroclor1254 or Phenobarbital. Metabolic activation systems based on chemically induced S9 have been widely applied in in vitro studies; e.g. the Salmonella mutagenicity test and the micronucleus test.

S9 is the supernatant of homogenized liver (in 0.25M saccharose) that has been centrifuged at 9000g for 10min. For gaining the liver mircrosomes the homogenized liver is centrifuged at 100000g for 1h.

For use in Ames test, the S9 standard mixture is: 4-10% S9 liver extract, 4mM NADP, 5mM Glucose-6-phosphat, 10mM Na/K Phosphate buffer (pH7.4), 8mM Mg-Aspartat, 33mM KCl

S9 Liver Extract (Rat/Hamster/Human) - Product Information (PDF, 307 KB)

A detailed description of all aspects of screening and regulatory genetox tests can be find in: Genetic Toxicology Testing – A Laboratory Manual, edited by Ray Proudlock, Academic Press 2016

Lyophilized S9 Rat Liver Extract

Lyophilized S9 is easy to handle and especially useful when an ultralow freezer is not available. It is ready to use after reconstitution with the label volume of cold, sterile, purified water.

Catalog no. Description Storage Size
11-01L.1 S9 SD rat liver Aroclor in KCl, lyoph. -20°C 1.1 ml/vial
11-01L.2 S9 SD rat liver Aroclor in KCl, lyoph. -20°C 2.1 ml/vial

Frozen S9 Rat/Hamster/Human Liver Extract

Catalog no. Description Storage Size
11-101.1 S9 SD rat liver Aroclor in KCl, frozen -80°C 1 ml/vial
11-101.2 S9 SD rat liver Aroclor in KCl, frozen -80°C 2 ml/vial
11-101.5 S9 SD rat liver Aroclor in KCl, frozen -80°C 5 ml/vial
11-102.5 S9 SD rat liver uninduced in KCl, frozen -80°C 5 ml/vial
11-105.1 S9 SD rat liver PB/BNF in KCl, frozen -80°C 1 ml/vial
11-105.2 S9 SD rat liver PB/BNF in KCl, frozen -80°C 2 ml/vial
11-105.5 S9 SD rat liver PB/BNF in KCl, frozen -80°C 5 ml/vial
11-115.5 S9 SD rat liver Ethanol in KCl, frozen -80°C 5 ml/vial
12-107.2 S9 F344 rat liver Aroclor in KCl, frozen -80°C 2 ml/vial
14-103.5 S9 Human liver pooled, frozen -80°C 5 ml/vial
14-201.1 Microsomes Human liver pooled, frozen -80°C 1 ml/vial
15-03S.5 S9 Golden Syrian Hamster liver Aroclor in KCl for modified Ames, frozen -80°C 5 ml/vial
15-104.5 S9 Golden Syrian Hamster liver uninduced in KCl, frozen -80°C 5 ml/vial
18-101.01 Microsomes SD rat liver Aroclor, frozen -80°C 0.1 ml/vial

Please note:
Please ask for availability of S9 Mouse!
Microsomes can be prepared according to your specifications.
Ready-to-use SD Rat S9 Mix MUTAZYME is available as well.

NEW: S9 Liver Extract of Rainbow Trout (Oncorhynchus mykiss)

TRINOVA BIOCHEM now offers cryopreserved pools of rainbow trout S9 fractions prepared and characterized in accordance with the OECD guideline 319B, manufactured in Germany. Each lot comes with a Certificate of Analysis (CoA) including pool size and SDS-PAGE analysis.

The OECD Test Guideline 319B “Determination of in vitro intrinsic clearance using rainbow trout liver subcellular fraction (RT-S9)” was released on June 25, 2018.

For more information, please download our Product Information below!

S9 Rainbow Trout Liver Extract- Product Information (PDF, 296 KB)