Immune Cell Functional Assays
Whether it is a simple lymphocyte proliferation assay, donor lymphocyte infusion (DLI) testing or mixed cultures, these assays are flexible for any laboratory.
NEW: ImmunoGlo™-96 TCP
ATP bioluminescence T-lymphocyte cell proliferation and viability assay kit
- To measure T-lymphocyte proliferation with a non-radioactive assay
- T-lymphocyte proliferation assay kits work right out of the box
- Equal or improved sensitivity to a radioactive marker and 10–100 times increased sensitivity than WST-1 or CSFE assays
- 3 assay kits available: Growth medium with IL-2, CD3 + CD28, or IL-2 + CD3 + CD28 for improved T-cell proliferation (see diagram)
- A standardized 5-days incubation period can be varied if required
- Readout has been validated, and can easily re-validated with the included standards and controls
T-lymphocyte proliferation with HemoGro™ growth medium +IMDM, +IL-2 alone, +CD3/CD28 or +IL-2/CD3/CD28 performed with ImmunoGlo™-96 TCP (culture period: 5 days)
| CATALOG NO. | DESCRIPTION | STORAGE | SIZE |
|---|---|---|---|
| K2-T-1H | ImmunoGlo™-96 TCP with IL-2 alone | -20°C | 1 x 96-well kit |
| KM1-TCS1-1H | ImmunoGlo™-96 TCP with CD3 + CD28 | -20°C | 1 x 96-well kit |
| KM1-TCS2-1H | ImmunoGlo™-96 TCP with IL-2 + CD3 + CD28 | -20°C | 1 x 96-well kit |
Kit contents:
- HemoGro™ Serum-free medium for Human cells containing requested cytokine cocktails
- ATP Standard
- ATP Controls
- ATP Enumeration reagent
- Sterile, 96-well culture plates for culturing cells
- Non-sterile, 96-well plates (for assay standardization)
- Sterile, adhesive foil covers to maintain sterility of unused wells
- Assay manual
ImmunoGlo™-96
ATP bioluminescence lymphocyte proliferation assay
- Lymphocyte activation
- Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc.
- Cellular immune response studies
- Single-cell, T-lymphocyte cloning studies
- Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use
- Effect of accessory (non T-cells) on T-cell induction
- Effect of co-stimulators on T-cell induction
| Catalog no. | Description | Storage | Size |
|---|---|---|---|
| KM1-LPA-1 | ImmunoGlo™-96 | -20°C | 1 x 96-well kit |
| KM1-LPA-2 | ImmunoGlo™-96 | -20°C | 2 x 96-well kit |
| KM1-LPA-4 | ImmunoGlo™-96 | -20°C | 4 x 96-well kit |
ImmunoGlo™-MLC
ATP bioluminescence 1- or 2-way mixed lymphocyte culture assay
- One-way mixed lymphocyte culture (MLC) / reaction (MLR)
- Two-way mixed lymphocyte culture (MLC) / reaction (MLR)
- Allogeneic mixed lymphocyte culture
- Cellular immune response
- Dendritic cell interactions
- Mesenchymal stem/stromal cell interactions
- Investigations of agents that activate/suppress MLC/MLR
| Catalog no. | Description | Storage | Size |
|---|---|---|---|
| KM1-MLC-1 | ImmunoGlo™-MLC | -20°C | 1 x 96-well kit |
| KM1-MLC-2 | ImmunoGlo™-MLC | -20°C | 2 x 96-well kit |
| KM1-MLC-4 | ImmunoGlo™-MLC | -20°C | 4 x 96-well kit |
ImmunoFLUOR™-96
Fluorescence lymphocyte proliferation assay
- Lymphocyte activation
- Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc.
- Cellular immune response studies
- Single-cell, T-lymphocyte cloning studies
- Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use
- Effect of accessory (non T-cells) on T-cell induction
- Effect of co-stimulators on T-cell induction
| Catalog no. | Description | Storage | Size |
|---|---|---|---|
| KM2-LPA-1 | ImmunoFLUOR™-96 | -20°C | 1 x 96-well kit |
| KM2-LPA-2 | ImmunoFLUOR™-96 | -20°C | 2 x 96-well kit |
| KM2-LPA-4 | ImmunoFLUOR™-96 | -20°C | 4 x 96-well kit |
ImmunoFLUOR™-MLC
Fluorescence 1- or 2-way mixed lymphocyte culture assay
- One-way mixed lymphocyte culture (MLC) / reaction (MLR)
- Two-way mixed lymphocyte culture (MLC) / reaction (MLR)
- Allogeneic mixed lymphocyte culture
- Cellular immune response
- Dendritic cell interactions
- Mesenchymal stem/stromal cell interactions
- Investigations of agents that activate/suppress MLC/MLR
| Catalog no. | Description | Storage | Size |
|---|---|---|---|
| KM2-MLC-1 | ImmunoFluor™-MLC | -20°C | 1 x 96-well kit |
| KM2-MLC-2 | ImmunoFluor™-MLC | -20°C | 2 x 96-well kit |
| KM2-MLC-4 | ImmunoFluor™-MLC | -20°C | 4 x 96-well kit |
ImmunoLIGHT™-96
Absorbance/colorimetric lymphocyte proliferation assay
- Lymphocyte activation
- Test unprimed T-cells in the presence of antibodies, enterotoxins, mitogens etc
- Cellular immune response studies
- Single-cell, T-lymphocyte cloning studies
- Test DLI (Donor Lymphocyte Infusion) samples for stimulation/induction prior to use
- Effect of accessory (non T-cells) on T-cell induction
- Effect of co-stimulators on T-cell induction
| Catalog no. | Description | Storage | Size |
|---|---|---|---|
| KM3-LPA-1 | ImmunoLIGHT™-96 | -20°C | 1 x 96-well kit |
| KM3-LPA-2 | ImmunoLIGHT™-96 | -20°C | 2 x 96-well kit |
| KM3-LPA-4 | ImmunoLIGHT™-96 | -20°C | 4 x 96-well kit |
ImmunoLIGHT™-MLC
Absorbance/colorimetric mixed lymphocyte culture assay
- One-way mixed lymphocyte culture (MLC) / reaction (MLR)
- Two-way mixed lymphocyte culture (MLC) / reaction (MLR)
- Allogeneic mixed lymphocyte culture
- Cellular immune response
- Dendritic cell interactions
- Mesenchymal stem/stromal cell interactions
- Investigations of agents that activate/suppress MLC/MLR
| Catalog no. | Description | Storage | Size |
|---|---|---|---|
| KM3-MLC-1 | ImmunoLIGHT™-MLC | -20°C | 1 x 96-well kit |
| KM3-MLC-2 | ImmunoLIGHT™-MLC | -20°C | 2 x 96-well kit |
| KM3-MLC-4 | ImmunoLIGHT™-MLC | -20°C | 4 x 96-well kit |
