In vitro Immunotoxicity Testing

Although often considered a separate area, immunotoxicity and hemotoxicity are intimately related since the immune and hematopoietic systems share common primitive stem cells. For the more mature immune cell populations Preferred Cell Systems has developed:

ImmunoGlo TCP

ATP bioluminescence T-lymphocyte cell proliferation and viability assay kits

  • Non-radioactive assay to measure T-lymphocyte proliferation
  • T-lymphocyte proliferation assay kits which work right out of the box
  • Equal or improved sensitivity to a radioactive marker and 10–100 times increased sensitivity than WST-1 or CSFE assays
  • 3 assay kits available: Growth medium with IL-2, CD3 + CD28, or IL-2 + CD3 + CD28 for improved T-cell proliferation (see diagram)
  • A standardized 5-days incubation period can be varied if required
  • Readout has been validated, and can easily re-validated with the included standards and controls

T-lymphocyte proliferation with HemoGro growth medium +IMDM, +IL-2 alone, +CD3/CD28 or +IL-2/CD3/CD28 performed with ImmunoGlo TCP (culture period: 5 days)

Comparison of the ImmunoGlo-96 TCP assay kit types

CATALOG NO. DESCRIPTION STORAGE SIZE
K2-T-1H ImmunoGlo TCP with IL-2 alone -20°C 1 x 96-well kit
KM1-TCS1-1H ImmunoGlo TCP with CD3 + CD28 -20°C 1 x 96-well kit
KM1-TCS2-1H ImmunoGlo TCP with IL-2 + CD3 + CD28 -20°C 1 x 96-well kit

Kit contents:

  • HemoGro Serum-free medium for Human cells containing requested cytokine cocktails
  • ATP Standard
  • ATP Controls
  • ATP Enumeration reagent
  • Sterile, 96-well culture plates for culturing cells
  • Non-sterile, 96-well plates (for assay standardization)
  • Sterile, adhesive foil covers to maintain sterility of unused wells
  • Assay manual

ImmunoGlo TCP - Flyer (PDF, 470 KB)

ImmunoGlo-Tox HT

Assays for in vitro immunotoxicity

This standardized ATP bioluminescence 96- or 384-well plate in vitro immunotoxicity testing system can be used to detect cytotoxicity associated with stimulator and responder cells.

CATALOG NO. DESCRIPTION STORAGE SIZE
KM1-T96-2 ImmunoGlo-Tox HT for user defined immune cells -20°C 2 x 96-well kit
KM1-T96-4 ImmunoGlo-Tox HT for user defined immune cells -20°C 4 x 96-well kit
KM1-T384-2 ImmunoGlo-Tox HT for user defined immune cells -20°C 2 x 384-well kit
KM1-T384-4 ImmunoGlo-Tox HT for user defined immune cells -20°C 4 x 384-well kit
KM1-T96CS1-2H ImmunoGlo-Tox HT with CD3 + CD28, Human -20°C 2 x 96-well kit
KM1-T384CS1-2H ImmunoGlo-Tox HT with CD3 + CD28, Human -20°C 2 x 384-well kit
KM1-T96CS2-2H ImmunoGlo-Tox HT with IL-2 + CD3 + CD28, Human -20°C 2 x 96-well kit
KM1-T384CS2-2H ImmunoGlo-Tox HT with IL-2 + CD3 + CD28, Human -20°C 2 x 384-well kit

ImmunoGlo Real Time

ATP bioluminescence stem and progenitor cell proliferation kit including standard and controls

  • Non-lytic, bioluminescence assay – follow lymphocyte proliferation in real time
  • Multiplexing flow cytometric capability
  • Add ImmunoGlo Real Time reagent at the beginning of culture or at any time after culture initiation, and follow cell growth for up to 3 day
  • Complete flexibility to use any mitogens, co-stimulators etc. and lymphocyte culture protocols
  • For use with peripheral blood mononuclear cells (PBMS), lymphocyte sub-populations and lymphocyte tissues and organs
CATALOG NO. DESCRIPTION STORAGE SIZE
KM1-RT1 ImmunoGlo Real Time -20°C 1 x 96-well kit