Instrument-based Proliferation Assays

Instead of detecting differentiation, CFU-equivalent assays directly measure cell proliferation of hematopoietic stem and progenitor cells. Proliferation occurs prior to differentiation and therefore results from CFU-equivalent assays are obtained already after 5-7 days instead of 10-14 days.

All our CFU-equivalent assays incorporate Suspension Expansion Culture (SEC) technology instead of methylcellulose. They are all instrument-based using either a bioluminescence, fluorescence or absorbance readout. SEC technology significantly reduces dispensing errors associated with methylcellulose, but also allows cell interactions to occur that results in shorter lag times to initiate proliferation.


Principle Bioluminescence:



Principle Bioluminescence

HALO® PCA

ATP bioluminescence CFU equivalent assay

Catalog no. Description Storage Size
K2-PCA1-1 HALO® PCA for SC-GEM2 / CFC-GEM2 -20°C 1 x 96-well kit
K2-PCA2-1 HALO® PCA for SC-GEM3 / CFC-GEM3 -20°C 1 x 96-well kit
K2-PCA3-1 HALO® PCA for P-GM1 / CFC-GM1 -20°C 1 x 96-well kit
K2-PCA4-1 HALO® PCA for P-GM2 / CFC-GM2 -20°C 1 x 96-well kit
K2-PCA5-1 HALO® PCA for SC-GEMM3 / CFC-GEMM3 -20°C 1 x 96-well kit

HALO® PCA is available in "Low serum" (see above) or "Serum-free" HemoGro high-performance culture growth medium, and in different package sizes.


HemoFLUOR PCA

Fluorescence CFU equivalent assay

Catalog no. Description Storage Size
K5-PCA1-1 HemoFLUOR PCA for SC-GEM2 / CFC-GEM2 -20°C 1 x 96-well kit
K5-PCA2-1 HemoFLUOR PCA for SC-GEM3 / CFC-GEM3 -20°C 1 x 96-well kit
K5-PCA3-1 HemoFLUOR PCA for P-GM1 / CFC-GM1 -20°C 1 x 96-well kit
K5-PCA4-1 HemoFLUOR PCA for P-GM2 / CFC-GM2 -20°C 1 x 96-well kit
K5-PCA5-1 HemoFLUOR PCA for SC-GEMM3 / CFC-GEMM3 -20°C 1 x 96-well kit

HemoFLUOR PCA is available in "Low serum" (see above) or "Serum-free" HemoGro high-performance culture growth medium, and in different package sizes.


HemoLIGHT PCA

Absorbance/colorimetric CFU equivalent assay

Catalog no. Description Storage Size
K4-PCA1-1 HemoLIGHT PCA for SC-GEM2 / CFC-GEM2 -20°C 1 x 96-well kit
K4-PCA2-1 HemoLIGHT PCA for SC-GEM3 / CFC-GEM3 -20°C 1 x 96-well kit
K4-PCA3-1 HemoLIGHT PCA for P-GM1 / CFC-GM1 -20°C 1 x 96-well kit
K4-PCA4-1 HemoLIGHT PCA for P-GM2 / CFC-GM2 -20°C 1 x 96-well kit
K4-PCA5-1 HemoLIGHT PCA for SC-GEMM3 / CFC-GEMM3 -20°C 1 x 96-well kit

HemoLIGHT PCA is available in "Low serum" (see above) or "Serum-free" HemoGro high-performance culture growth medium, and in different package sizes.


HALO® Real Time

Real time, non-lytic bioluminescence assay to monitor cell processing of hematopoietic stem/progenitor cells

  • Non-lytic, bioluminescence assay. Compatible with downstream applications, e.g flow cytometric phenotypic analysis (e.g. CD133+, CD34+ or ALDH+)
  • Watch the cells grow over 2-5 days by taking bioluminescence readings at any times you like
  • For human cells, bone marrow, umbilical cord blood normal or mobilized peripheral blood or purified populations (e.g. CD34+, CD133+)
  • Available for 7 lympho-hematopoietic stem cell and 9 progenitor cell populations
Catalog no. Description Storage Size
K6-HPP1-1H HALO® Real Time for SC-HPP1 / HPP-SP1 -20°C 1 x 96-well kit
K6-HPP2-1H HALO® Real Time for SC-HPP2 / HPP-SP2 -20°C 1 x 96-well kit
K6-GEMM1-1H HALO® Real Time for SC-GEMM1 / CFC-GEMM1 -20°C 1 x 96-well kit
K6-GEMM2-1H HALO® Real Time for SC-GEMM2 / CFC-GEMM2 -20°C 1 x 96-well kit
K6-GEM1-1H HALO® Real Time for SC-GEM1 / CFC-GEM1 -20°C 1 x 96-well kit
K6-GEM2-1H HALO® Real Time for SC-GEM2 / CFC-GEM2 -20°C 1 x 96-well kit
K6-BFU1-1H HALO® Real Time for P-BFU1 / BFU-E1 -20°C 1 x 96-well kit
K6-BFU2-1H HALO® Real Time for P-BFU2 / BFU-E2 -20°C 1 x 96-well kit
K6-GM1-1H HALO® Real Time for P-GM1 / GM-CFC1 -20°C 1 x 96-well kit
K6-GM3-1H HALO® Real Time for P-GM3 / GM-CFC3 -20°C 1 x 96-well kit
K6-Mk1-1H HALO® Real Time for P-Mk1 / Mk-CFC1 -20°C 1 x 96-well kit

HALO® Real Time is available either "Serum-free", or "Low serum" (see above) combined with the following species: Human (see above), Primate, Rat, Mouse and Canine.


HALO® Real Time- Flyer (PDF, 642 KB)
HALO Assays - Flyer (PDF, 611 KB)

Please find Terminology / Synonyms of hematopoietic stem, progenitor and precursor cells here