Instrument-based Proliferation Assays for Hemapoietic Stem and Progenitor Cells

Preferred Cell Systems assays allow you to measure proliferation and differentiation of various stem and progenitor cells. Choose from a variety of readouts including:

  • Bioluminescence
  • Flourescence
  • Absorbance

Bioluminescence principle



Principle Bioluminescence
Correlation of proliferation (ATP bioluminescence) and mean total colony number
Correlation proliferation-colony number with CAMEO-4 assay



HALO®

ATP bioluminescence proliferation and viability assays including standard and controls

Catalog no. Description Storage Size
K2-0-1H HALO® without growth factors -20°C 1 x 96-well kit
K2-HPP1-1H HALO® for SC-HPP1 / HPP-SP1 -20°C 1 x 96-well kit
K2-HPP2-1H HALO® for SC-HPP2 / HPP-SP2 -20°C 1 x 96-well kit
K2-GEMM1-1H HALO® for SC-GEMM1 / CFC-GEMM1 -20°C 1 x 96-well kit
K2-GEMM2-1H HALO® for SC-GEMM2 / CFC-GEMM2 -20°C 1 x 96-well kit
K2-GEM1-1H HALO® for SC-GEM1 / CFC-GEM1 -20°C 1 x 96-well kit
K2-GEM2-1H HALO® for SC-GEM2 / CFC-GEM2 -20°C 1 x 96-well kit
K2-BFU1-1H HALO® for P-BFU1 / BFU-E1 -20°C 1 x 96-well kit
K2-BFU2-1H HALO® for P-BFU2 / BFU-E2 -20°C 1 x 96-well kit
K2-GM1-1H HALO® for P-GM1 / GM-CFC1 -20°C 1 x 96-well kit
K2-GM3-1H HALO® for P-GM3 / GM-CFC3 -20°C 1 x 96-well kit
K2-Mk1-1H HALO® for P-Mk1 / Mk-CFC1 -20°C 1 x 96-well kit
K2-T-1H HALO® for P-Tcell / T-CFC -20°C 1 x 96-well kit
K2-B-1H HALO® for P-Bcell / B-CFC -20°C 1 x 96-well kit

Kits are available for following species: Human (see above), Primate, Equine, Sheep, Porcine, Rat, Mouse and Canine cells, and "Serum-free" for Human, Primate and Mouse cells.



Comparison between Human tissues in the response of 7 cell populations detected using HALO®

Human tissue comparison with HALO kit

HemoFLUOR

Fluorescence proliferation and viability assays

Catalog no. Description Storage Size
K5-0-1H HemoFLUOR without growth factors -20°C 1 x 96-well kit
K5-HPP1-1H HemoFLUOR for SC-HPP1 / HPP-SP1 -20°C 1 x 96-well kit
K5-HPP2-1H HemoFLUOR for SC-HPP2 / HPP-SP2 -20°C 1 x 96-well kit
K5-GEMM1-1H HemoFLUOR for SC-GEMM1 / CFC-GEMM1 -20°C 1 x 96-well kit
K5-GEMM2-1H HemoFLUOR for SC-GEMM2 / CFC-GEMM2 -20°C 1 x 96-well kit
K5-GEM1-1H HemoFLUOR for SC-GEM1 / CFC-GEM1 -20°C 1 x 96-well kit
K5-GEM2-1H HemoFLUOR for SC-GEM2 / CFC-GEM2 -20°C 1 x 96-well kit
K5-BFU1-1H HemoFLUOR for P-BFU1 / BFU-E1 -20°C 1 x 96-well kit
K5-BFU2-1H HemoFLUOR for P-BFU2 / BFU-E2 -20°C 1 x 96-well kit
K5-GM1-1H HemoFLUOR for P-GM1 / GM-CFC1 -20°C 1 x 96-well kit
K5-GM3-1H HemoFLUOR for P-GM3 / GM-CFC3 -20°C 1 x 96-well kit
K5-Mk1-1H HemoFLUOR for P-Mk1 / Mk-CFC1 -20°C 1 x 96-well kit
K5-T-1H HemoFLUOR for P-Tcell / T-CFC -20°C 1 x 96-well kit
K5-B-1H HemoFLUOR for P-Bcell / B-CFC -20°C 1 x 96-well kit

Available for following species: Human (see above), Primate, Equine, Sheep, Porcine, Rat, Mouse and Canine cells, and "Serum-free" for Human, Primate and Mouse cells.


HemoLIGHT

Absorbance/colorimetric proliferation and viability assays

Catalog no. Description Storage Size
K4-0-1H HemoLIGHT without growth factors -20°C 1 x 96-well kit
K4-HPP1-1H HemoLIGHT for SC-HPP1 / HPP-SP1 -20°C 1 x 96-well kit
K4-HPP2-1H HemoLIGHT for SC-HPP2 / HPP-SP2 -20°C 1 x 96-well kit
K4-GEMM1-1H HemoLIGHT for SC-GEMM1 / CFC-GEMM1 -20°C 1 x 96-well kit
K4-GEMM2-1H HemoLIGHT for SC-GEMM2 / CFC-GEMM2 -20°C 1 x 96-well kit
K4-GEM1-1H HemoLIGHT for SC-GEM1 / CFC-GEM1 -20°C 1 x 96-well kit
K4-GEM2-1H HemoLIGHT for SC-GEM2 / CFC-GEM2 -20°C 1 x 96-well kit
K4-BFU1-1H HemoLIGHT for P-BFU1 / BFU-E1 -20°C 1 x 96-well kit
K4-BFU2-1H HemoLIGHT for P-BFU2 / BFU-E2 -20°C 1 x 96-well kit
K4-GM1-1H HemoLIGHT for P-GM1 / GM-CFC1 -20°C 1 x 96-well kit
K4-GM3-1H HemoLIGHT for P-GM3 / GM-CFC3 -20°C 1 x 96-well kit
K4-Mk1-1H HemoLIGHT for P-Mk1 / Mk-CFC1 -20°C 1 x 96-well kit
K4-T-1H HemoLIGHT for P-Tcell / T-CFC -20°C 1 x 96-well kit
K4-B-1H HemoLIGHT for P-Bcell / B-CFC -20°C 1 x 96-well kit

Available for following species: Human (see above), Primate, Equine, Sheep, Porcine, Rat, Mouse and Canine cells, and "Serum-free" for Human, Primate and Mouse cells.


CAMEO-96

Combined CFU & Bioluminescence assays

Methylcellulose assay for manual colony enumeration AND standardized ATP-bioluminescence

Catalog no. Description Storage Size
KCO-0-1H CAMEO-96 without growth factors -20°C 1 x 96-well kit
KCO-HPP1-1H CAMEO-96 for SC-HPP1 / HPP-SP1 -20°C 1 x 96-well kit
KCO-HPP2-1H CAMEO-96 for SC-HPP2 / HPP-SP2 -20°C 1 x 96-well kit
KCO-GEMM1-1H CAMEO-96 for SC-GEMM1 / CFC-GEMM1 -20°C 1 x 96-well kit
KCO-GEMM2-1H CAMEO-96 for SC-GEMM2 / CFC-GEMM2 -20°C 1 x 96-well kit
KCO-GEM1-1H CAMEO-96 for SC-GEM1 / CFC-GEM1 -20°C 1 x 96-well kit
KCO-GEM2-1H CAMEO-96 for SC-GEM2 / CFC-GEM2 -20°C 1 x 96-well kit
KCO-BFU1-1H CAMEO-96 for P-BFU1 / BFU-E1 -20°C 1 x 96-well kit
KCO-GM1-1H CAMEO-96 for P-GM1 / GM-CFC1 -20°C 1 x 96-well kit
KCO-Mk1-1H CAMEO-96 for P-Mk1 / Mk-CFC1 -20°C 1 x 96-well kit
KCO-T-1H CAMEO-96 for P-Tcell / T-CFC -20°C 1 x 96-well kit
KCO-B-1H CAMEO-96 for P-Bcell / B-CFC -20°C 1 x 96-well kit

Available for following species: Human (see above), Primate, Equine, Sheep, Porcine, Rat, Mouse and Canine cells.


HALO® Real Time

Real time, non-lytic bioluminescence assays to monitor cell processing of hematopoietic stem/progenitor cells

  • Non-lytic, bioluminescence assay. Compatible with downstream applications, e.g. flow cytometric phenotypic analysis (e.g. CD133+, CD34+ or ALDH+)
  • Watch the cells grow over 2-5 days by taking bioluminescence readings at any times you like
  • For human cells, bone marrow, umbilical cord blood normal or mobilized peripheral blood or purified populations (e.g. CD34+, CD133+)
  • Available for 7 lympho-hematopoietic stem cell and 9 progenitor cell populations
Catalog no. Description Storage Size
K6-HPP1-1H HALO® Real Time for SC-HPP1 / HPP-SP1 -20°C 1 x 96-well kit
K6-HPP2-1H HALO® Real Time for SC-HPP2 / HPP-SP2 -20°C 1 x 96-well kit
K6-GEMM1-1H HALO® Real Time for SC-GEMM1 / CFC-GEMM1 -20°C 1 x 96-well kit
K6-GEMM2-1H HALO® Real Time for SC-GEMM2 / CFC-GEMM2 -20°C 1 x 96-well kit
K6-GEM1-1H HALO® Real Time for SC-GEM1 / CFC-GEM1 -20°C 1 x 96-well kit
K6-GEM2-1H HALO® Real Time for SC-GEM2 / CFC-GEM2 -20°C 1 x 96-well kit
K6-BFU1-1H HALO® Real Time for P-BFU1 / BFU-E1 -20°C 1 x 96-well kit
K6-BFU2-1H HALO® Real Time for P-BFU2 / BFU-E2 -20°C 1 x 96-well kit
K6-GM1-1H HALO® Real Time for P-GM1 / GM-CFC1 -20°C 1 x 96-well kit
K6-GM3-1H HALO® Real Time for P-GM3 / GM-CFC3 -20°C 1 x 96-well kit
K6-Mk1-1H HALO® Real Time for P-Mk1 / Mk-CFC1 -20°C 1 x 96-well kit

HALO® Real Time is available either "Serum-free" (see above) or "Low serum", combined with the following species: Human (see above), Primate, Rat, Mouse and Canine.


HALO® Real Time- Flyer (PDF, 642 KB)

Please find Terminology / Synonyms of hematopoietic stem, progenitor and precursor cells here