Ames Plate Incorporation Assay Kits

Salmonella Mutagenicity Complete Test Kit

Ames plate

Provides all essential items for conducting the bacterial mutagenesis assay described by Maron and Ames1

The Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 are included in the kit. The lyophilized strains are fully characterized for phenotypic characteristics and respond to diagnostic mutagens as required by OECD Guideline 4713.

Kit contents:

  • STDisc TA98, TA100, TA1535, TA1537 (10 discs per strain/vial)
  • Lyophilized S9, Aroclor induced (2 vials/2.1 ml), optional: PB/BNF induced S9
  • NADPH Regensys (50 ml Regensys A and 50 ml Regensys B)
  • CONTROLCHEM: Mitomycin C, ICR191, Daunomycin, Sodium azide & 2-Aminoanthracene
  • Nutrient broth, Histidine/Biotin Top Agar, ST QUA PC plates
  • Minimal Glucose Agar (MGA) plates (160 dishes), Nutrient Agar plates (20 dishes)
  • Application and instruction manual
Catalog no. Description Storage Size
31-100.2 Salmonella Mutagenicity Complete Test Kit - 1 sample kit

This kit can be upgraded by substitution or addition of our E. coli or Salmonella typhimurium strains to comply with the OECD Guideline 4713. For high-throughput-screening please ask for bulk ware.

E. coli Mutagenicity Complete Test Kit

Mutagenicity

Provides all essential items for conducting the bacterial mutagenesis assay described by Green and Muriel2

Escherichia coli strains ECDisc WP2 trp and WP2 trp, uvrA are included in the kit. The lyophilized strains are fully characterized for phenotypic characteristics and respond to diagnostic mutagens as required by OECD Guideline 4713.

Kit contents:

  • ECDisc WP2 trp and WP2 trp, uvrA (10 discs per strain/vial)
  • Lyophilized S9, Aroclor induced (2 vials/2.1 ml), optional: PB/BNF induced S9
  • NADPH Regensys (15 ml Regensys A and 15 ml Regensys B)
  • CONTROLCHEM: Methylmethanesulfonate, 2-Aminoanthracene
  • Nutrient broth, L-Tryptophan Top Agar, EC TRI PC plates
  • Minimal Glucose Agar (MGA) plates (80 dishes), Nutrient Agar plates (20 dishes)
  • Application and instruction manual
Catalog no. Description Storage Size
31-101 E. coli Mutagenicity Complete Test Kit - 1 sample kit

µAmes Bacterial Mutation Test Kit

µAmes 24-well plate

24-well plate format, miniaturized Ames bacterial mutation test

The µAmes test allows Ames testing compliant with OECD guideline 4713 and is suitable to evaluate mutagenicity of pharmaceutical impurities when only limited amounts are available (see Guideline:ICH M74).

Advantages of the µAmes method:

  • 95% less test article is required
  • One technician may perform the test in a single day with results 3 days later
  • Each bacterial strain is purified, fully characterized, lyophilized and provided with a quality control and formulation statement
  • Phenotype-testing components confirm the characteristics of the cultures generated in your lab

Kit contents:

  • Lyophilized strains Salmonella TA98, TA100, TA1535, TA1537 and E. coli WP2 trp, uvrA
  • 10% S9 Mix, lyophilized (MUTAZYME)
  • 24-well plates with Minimal 0.4% Glucose Agar
  • CONTROLCHEM pre-weighted positive control chemicals: Sodium azide, 9-Aminoacridine, 2-Nitrofluorene, 4-Nitroquinoline-1-oxide, 2-Aminoanthracene, Benz(a)pyrene
  • Nutrient broth, Phosphate buffer, Top Agar His/Bio/Tryp
  • Phenotype confirmation plates and 4 types of antibiotic discs
  • Spectrometer cuvettes, sterile 50ml tubes, sterile microfuge tubes and loops
  • Application and instruction manual
Catalog no. Description Storage Size
31-500 µAmes Bacterial Mutation Test Kit - 1 sample kit
µAmes Assay Kit- Product Information (PDF, 300 KB)

1 Maron D., Ames B. Revised methods for the Salmonella mutagenicity test. Mutation Research 113(3-4):173-215, 1983.
2 Green M.H., Muriel W.J. Mutagen testing using TRP+ reversion in Escherichia coli. Mutation Research 38(1):3-32, 1976.
3 OECD Guideline 471 for the Testing of Chemicals, Section 4. Test No. 471: Bacterial Reverse Mutation Test
4 Guideline: ICH M7 Assessment and Control of DNA reactive (mutagenic) Impurities in Pharmaceuticals to limit potential carcinogenic risk